Patricia Coltri - USP

In our lab, we are interested on splicing regulation in eukaryotes. Control of gene expression depends on extensive RNA processing. Most genes are composed by exons and introns. Splicing is the removal of intervening sequences, or introns, and ligation of exons into mature RNAs. These RNAs can be directed to translation or, in some cases, perform other roles in the cell. This process is performed by a complex macromolecular complex called the spliceosome

The spliceosome is a multi-megadalton machinery assembled on every RNA transcript. It is composed of five small nuclear RNAs (U1, U2, U4, U5 and U6 snRNAs) and over 100 different proteins. Most of these are core components of the complex whereas some are transiently associated regulatory factors.

We investigate the mechanisms that direct and guide the splicing machinery to the transcript. We use molecular and cell biology techniques to understand how this activity is modulated by different proteins and spliceosome components as well as by different sequences on transcripts.

If you are interested in joining us, please send your info to: coltri [at] usp [dot] br





Lab members





Gisela T. Ramos, technician





Dr. Rosicler Lázaro Barbosa

Identification and characterization of Trypanosoma spliceosomes isolated under different conditions.



Graduate students



Guilherme Henrique Gatti da Silva

Regulation of eukaryotic miRNA splicing
Funding: CAPES



Undergraduate students


Arthur Tributino Menezes

GFP reporter transcript construction and splicing analysis in human cells.


Helder Nagasse

Splicing efficiency in miRNA-containing transcripts



Former students


Juliana Pimentel Galhardo


Marcelo Machado Paiva